LIU JUNLING

Liu Junling

Professor Liu is a graduate of the Beijing University of Chinese Medicine and School of China Academy of Medical Sciences.  He has undertaken a number of studies including "effects of acupuncture on cardiac activities and its neurobiological mechanism" and "mechanisms of the accumulative effect of repeated acupuncture in relieving chronic pain".  He is currently a Professor at the Institute of Acupuncture & Moxibustion, China Academy of Chinese Medical Sciences.

1- gera: 9671/di/ra
 [ROLE DE L’HYPOTHALAMUS DANS LE TRAITEMENT PAR ACUPUNCTURE DE L’ISCHEMIE AIGUE CHEZ LE LAPIN. 2) EFFETS DE LA STIMULATION DE L’AIRE*]. LIU JUNLING. journal of traditional chinese medicine. 1984;4(3):197-204 (eng).
  
2- gera: 9670/di/ra
 [ROLE DE L’HYPOTHALAMUS DANS LE TRAITEMENT PAR ACUPUNCTURE DE L’ISCHEMIE AIGUE MYOCARDIQUE CHEZ LE LAPIN (1)]. LIU JUNLING ET AL. journal of traditional chinese medicine. 1984;4(2):119-6 (eng).
 
 3- gera: 22985/di/cg
 EFFECT OF HYPOTHALAMUS ON THE RECOVERY OF ACUTE ISCHEMIC MYOCARDIAL INJURY PROMOTED BY  ELECTROACUPUNCTURE IN RABBITS : *. LIU JUNLING ET AL. second national symposium on acupuncture, beijing. 1984;:502 (eng).
 
 4- gera: 22990/di/cg
 EFFECT OF HYPOTHALAMUS ON THE RECOVERY OF ACUTE ISCHEMIC MYOCARDIAL INJURY PROMOTED BY  ELECTROACUPUNCTURE IN RABBITS*. LIU JUNLING ET AL. second national symposium on acupuncture, beijing. 1984;:503 (eng).
 
 5- gera: 25122/di/cg
 EFFECT OF ELECTROACUPUNCTURE AT "NEIGUAN" ON THE ELECTRICAL ACTIVITIES OF NEURONS IN POSTERIOR-HYPOTHALAMIC AREA INDUCED BY ACUTE MYOCARDIAL ISCHEMIA. LIU JUNLING ET AL. selections from article abstracts on acupuncture and moxibustion, beijing. 1987;:431 (eng).
 
 6- gera: 25259/di/cg
 EFFECT OF ELECTROACUPUNCTURE AT "NEIGUAN" ON THE SPONTANEOUS ELECTRICAL ACTIVITIES OF THE NEURONS IN PREOPTIC-ANTERIOR HYPOTHALAMUS. LIU JUNLING ET AL. selections from article abstracts on acupuncture and moxibustion, beijing. 1987;:577 (eng).
 
 7- gera: 53850/di/ra
 [INFLUENCE OF THORACIC SPINAL SUBARACHNOID MICROINJECTION OF PHENTOLAMINE ON THE EFFECT OF ELECTROACUPUNCTURE]. LIU JUNLING ET AL. acupuncture research. 1994;19(2):47-51 (chi*).
 The influence of thoracic spinal subarachnoid microinjection of Phentolamine (Phen, 200ug/20ul) on the effect of electroacupuncture (EA) at Neiguan (PC 6) was observed in 42 urethan-chloralose anesthetized rabbits. Results showed that: 1) EA of Neiguan (PC 6) could significantly promote recovery of elevated ST-segments and T-waves of electrocardiogram induced by acute myocardial ischemia (AMI). 2) subarachnoid microinjection of phentolamine had no marked influence on recovery of ST-segments and T-waves, but impeded recovery of blood pressure of post-AMI; and 3) subarachnoid microinjection of phentolamine could significantly weaken or eliminate the effect of EA of Neiguan (PC 6) in accelerating recovery of ST-segments and T-waves of post-AMI. It denotes that alpha-receptors of intra-thoracic spinal cord participate in the action of EA of Neiguan (PC 6) in improving AMI, and the upper thoracic segment of the spinal cord is one of the links for connecting Neiguan and the heart.
 
8- gera: 85695/di/ra
 [INFLUENCE OF THORACIC SPINAL SUBARACHNOID MICROINJECTION OF NORADRENALINE ON THE EFFECT OF ELECTROACUPUNCTURE]. LIU JUNLING ET AL. acupuncture research. 1995;20(2):28-32 (chi*).
 Influence of thoracic spinal subarachnoid microinjection of noradrenaline (NA, 40mg/20ul) on the effect of electroacupuncture (EA) at Neiguan (PC6) was observed in 40 rabbits anesthetized with urethan-chloralose. Results showed that : 1) EA of Neiguan (PC6) could significantly accelerate the recovery of ST-segment and T-wave of electrocardiogram induced by acute myocardial ischemia (AMI); 2). Subarachnoid microinjection of NA also could promote the recovery of ST,T and blood pressure of post-AMI; and 3) Subarachnoid microinjection of NA could strengthen the effect of EA of Neiguan (PC6) to Promote the recovery of ST and T of Post-AMI. It suggests that alpha-receptors of intra-thoracic spinal cord participate in the action of EA of Neiguan (PC6) ameliorating AMI, and the upper thoracic segment is one of the links for connecting Neiguan and the heart.
 
9- gera: 55403/di/ra
 [INFLUENCE OF NEURONAL EXCITATION AND INHIBITION OF ROSTRAL VENTROLATERAL MEDULLA ON THE EFFECT OF ELECTROACUPUNCTURE OF "NEIGUAN" ACUPOINT]. LIU JUNLING ET AL. acupuncture research. 1996;21(1):34-8 (chi*).
 
 10- gera: 55420/di/ra
 [INFLUENCE OF MICROINJECTION OF CLONIDINE AND YOHIMBINE IN rVLM ON THE EFFECT OF ELECTROACUPUNCTURE TREATMENT OF MYOCARDIAL ISCHEMIA]. LIU JUNLING ET AL. acupuncture research. 1996;21(2):31-5 (chi*).
 
 11- gera: 85766/di/ra
 [EFFECTS OF ELECTROACUPUNCTURE AT POINTS AND NON-POINTS OF THE PERICARDIUM CHANNEL ON ELECTRIC ACTIVITY OF ISCHEMIC MYOCARDIUM IN RABBITS]. LIU JUNLING ET AL. chinese acupuncture and moxibustion. 1996;16(4):31 (chi*).
 Effects of electroacupuncture at "Neiguan" (PC5), "Zhongchong (PC9), Laogong (PC8), Daling (PC7)" points and non-acupoints of the pericardium channel on electric activity of ischemic myocardium in 44 anesthetic rabbits. Results indicated that electroacupuncture at the points and the non-acupoints on the pericardium channel all could markedly promote recovery of S-T segment of ECG and the mean arterial pressure after ischemia, inhibit reduction of MAPA and accelerate restoration of MAPD50 and MAPD90. It is suggested that electroacupuncture at the points and the non-acupoints of the pericardium channel can maintain relative stability of electric activity of ischemic myocardium, improve function of pumping blood and accelerate recovery of blood pressure. Also it is indicated that both the point and non-acupoint on the pericardium channel are related closely with the heart.
 
12- gera: 67173/di/ra
 EFFECT OF ALPHA 1 AND ALPHA 2 RECEPTORS IN THE RABBIT THORACIC SPINAL CORD ON ELECTROACUPUNCTURE AT "NEIGUAN" FOR TREATMENT OF ACUTE MYOCARDIAL ISCHEMIA. LIU JUNLING ET AL. world journal of acupuncture-moxibustion. 1998;8(4):28-34 (eng).
 In the present paper, the effect of alpha1 and alpha2 subtypes of the thoracic spinal cord on electroacupuncture (EA)-induced improvement of ischemic cardiac electrical and mechanical activities was observed in 80 anesthetized rabbits by using ECG-ST, MBP, LVP and dp/dt max as indexes. Results showed that: 1) EA at "Neiguan" could significantly improve the electrical and mechanical activities of the ischemic heart; 2) the effect of EA could be enhanced to a certain degree when alpha1 receptors of the thoracic spinal cord were activated by subarachnoid microinjection of phenylephrine, while it was weakened when alpha1 receptors were inhibited by microinjection of parison; and 3) activation or inhibition of alpha2 receptors of the thoracic spinal cord by microinjection of clonidine and yohimbine had no marked influence on the effect of EA in improving electrical activity of the ischemic heart, but when activated, they could weaken the effect of EA in raising MBP; while suppressed, they had no any striking influence on the effect of EA in raising MBP. It suggests that among alpha- receptors, predominantly alpha1-receptors participate in the process of EA-induced improvement of performance of
 
13- gera: 77086/di/ra
 [STUDY ON MECHANISMS OF ELECTROACUPUNCTURE AT ACUPOINTS OF PERICARDIUM CHANNEL FOR IMPROVING EXPERIMENTAL ACUTE MYOCARDIAL ISCHEMIA]. LIU JUNLING ET AL. acupuncture research. 1999;24(4):282 (chi*).
 In the present study, the mechanisms of electroacupuncture (EA) at acupoints of the Pericardium Channel (PC) for improving experimental acute myocardial ischemia (AMI) were studied in rabbits, cats and rats, from the view of combination of physiological functions and anatomical structures and by using multiple indexes. Results demonstrated that: 1) EA of "Neiguan" (PC6), etc, and non-points of the PC was of favorable regulative action on the electrical and mechanical activities of the ischemic heart; the PC is a functional entirety in regulating physiological activity of the ischemic heart; the effect of acupoints of PC has a relative specificity to a certain degree; 2) EA could improve cardiac performance and myocardial energy metabolism, while its key action mainly lay in the improvement of the microcirculation of the ischemic myocardium; 3) the action of EA of PC acupoints in bettering AMI is closely associated with EA-induced inhibition on the hyperactivity of the periphery sympathy- adrenal gland-CA system, central adrenergic and noradrenergic systems during AMI, and also associated with EA- induced enhancement of functional activity of the adrenal cortex and increase in the organic anti-disease ability. Therefore, the local actions of EA on ameliorating AMI, protecting myocardial cells from injury are definitely not separable from its regulative action on the functional activities of the whole body. This fact suggests that in treatment of coronary heart disease, both the performance of the cardiovascular system and the state of the whole body should be considered simultaneously.
 
14- gera: 103827/di/ra
 WFAS 2001 INTERNATIONAL SYMPOSIUM ON ACUPUNCTURE WAS HELD IN SINGAPORE THEY STILL. LIU JUNLING . world journal of acupuncture-moxibustion . 2001;11(4):63 (eng).
 
 15- gera: 111830/di/ra
 ACTION OF DIFFERENT CERVICAL AND THORACIC DORSAL ROOTS IN CONVEYING ELECTROACUPUNCTURE SIGNALS FROM ACUPOINTS OF PERICARDIUM CHANNELS TO THE SPINAL CORD. LIU JUNLING ET AL. world journal of acupuncture-moxibustion. 2001;11(2):15 (eng*).
 
 16- gera: 114821/di/ra
 ACTION OF DIFFERENT CERVICAL AND THORACIC DORSAL ROOTS IN CONVEYING ELECTROACUPUNCTURE SIGNALS FROM ACUPOINTS OF PERICARDIUM CHANNELS TO THE SPINAL CORD. LIU JUNLING ET AL. world journal of acupuncture-moxibustion. 2001;11(2):15 (eng*).
 Objective : : To compare the effect of different cervical and thoracic dorsal roots in conveying electroacupuncture (EA) signals from acupoints of Pericardium Channel (PC) and Sanjiao Channel (SC) to the spinal cord for analyzing the relative specificity of the connection between PC and the heart. Methods : Experiments were conducted on 85 Wistar rats anesthetized with mixture solution of chloralose (5 mg/100 g body weight) and urethane (42 mg/100 mg). After laminectomy, the cervical and thoracic segments of the spinal cord were exposed. Under microscopic observation, the micro-filaments of the proximal end of the dorsal root were carefully isolated after tearing the spinal pia mater, and attached to the recording electrode. The reference electrode was placed beneath the skin of the incision. Two caudal and two rostral dorsal roots near the recorded dorsal root were cut off separately. Electrical activity of the isolated micro-filaments was observed and analyzed by using VC-10 Memory Oscilloscope and histogram. "Daling (PC 7) – Jianshi (PC 5)", Quze (PC 3) of PC, "Yangchi (TE 4) – Zhigou (TE 6)", "Tianjing (TE 10) – Qinglengyuan (TE 11)" of the SC were chosen and stimulated electrically with 1 and 2 mA, frequency of 40 Hz and intermittent waves. Results : (1) Effect of EA of PC acupoints on the firing of micro- filaments The effect of EA of PC acupoints on the electrical activity was observed in 60 micro-filaments of C-5-8 and TI dorsal roots. After EA stimulation of "Daling (PC 7) -Jianshi (PC 5), 3/12 C5, 5/12 C6, 7/13 C7, 3/12 C8 and 2/11 T, nerve micro-filaments increased considerably in the firing rate. After EA stimulation of " Quze (PC 3) v 3 C5, 4 %, 6 C7, 3 C8 and 3 T, of each 10 nerve micro-filaments increased significantly in the firing rate. It indicates that C7 dorsal root plays the first important role in conveying EA signals from PC acupoints to the spinal cord and higher central nerve system; (2) Effect of EA of TE Channel acupoints on the firing of nerve filaments After EA "Yangchi (TE 4) – Zhigou (TE 6) ", 2/10 C5, 7/10 C6, 5 of/C7, 1/10 C8, 2/13 T, nerve filaments increased significantly in the firing rate. After EA of "Tianjing (TE 10) – Qinglengyuan (TE 11)", 2 C5, 3 C6, 4 C7, 1 C8 and 1 T, of each 10 nerve micro-filaments increased strikingly in the discharge rate. It showed that EA signals of TE acupoints transmitted to the spinal cord mainly by way of C6 and C7 dorsal roots. Most of the above-mentioned nerve micro-filaments responding obviously to EA stimulation were low-threshold nerve fibers. 18 of 20 micro- filaments in "Daling-Jianshi" group and 15 of 17 micro-filaments in "Yangchi – Zhigou" group responded considerably to touch or slightly clamping the skin in the receptive field. Only 1 of 20 micro-filaments (C7) in
quot;Daling – Jian
hi" group and 2 of 17 filaments – (C7 and C8) in "Yangchi – Zhigou" group responded to pain stimulation within the receptive field. Conclusion :The periphery afferent pathway for transmitting signals from EA

 
17- gera: 104423/di/ra
 [PROGRESSES IN THE STUDY ON MERIDIAN THEORY IN 2000]. LIU JUNLING, CHEN ZHENRONG, HU XIANGLONG, ET AL. acupuncture research. 2001;26(1):72 (chi*).
 
 18- gera: 111952/di/ra
 OBSERSERVATION ON THE EFFECT OF ELECTROACUPUNCTURE ON ISCHEMIC CARDIAC FUNCTION IN THE INTACT AND SPINALIZED RABBITS. LIU JUNLING ET AL. world journal of acupuncture-moxibustion. 2002;12(4):28 (eng*).
 
 19- gera: 108185/di/ra
 [ADVANCES IN THE RESEARCH ON MERIDIANS-ONE OF THE PRELIMINARILY-SELECTED ITEMS OF THE STATE SCALING PROGRAM IN "THE 9TH FIVE-YEARS PERIOD"]. LIU JUNLING, CHEN ZHENRONG. acupuncture research. 2002;27(3):230 (chi*).
 
 20- gera: 106041/di/ra
 [RESEARCH ON THE CONNECTION PATHWAY BETWEEN " TIANQUAN " (PC 2) OF THE " PERICARDIUM. MERIDIAN" AND THE HEART IN THE CAT]. ZHANG JIANLIANG, CHEN SHUPING, LIU JUNLING. acupuncture research. 2002;27(2):124 (chi).
 Objective : To explore the connection pathway between 11 Tianquan" (PC 2) of the Pericardium Meridian and the heart with the combined electrophysiological and morphological techniques. Methods : 34 cats anesthetized with urethane (500 mg/kg) and chloralose (50 mg/kg) were used in the first part of the present study. (I) After isolating the left inferior cardiac nerve (LICN) and the left lateral cutaneous nerve (LLCN) and performing a skin flap bath of paraffin-oil, discharges of LCN were fed to VC-10 oscilloscope and histogramer via a bipolar platinum electrode (reference electrode placed beneath the subcutaneous tissues), and recorded by using a X-Y recorder. LICN was stimulated with an electric stimulator. The myoelectrical activities of "Tianquan" (PC 2) and "Tianfu" (LU 3) areas were recorded respectively through two pairs of acupuncture needle electrodes. The reaction threshold was set to that the electric stimulation just could evoke myoelectric signal changes. (2) In the second part of this study, ten cats anesthetized with 1 % pentobarbital (40 mg/kg) were used. Propidium Iodide (PI, 10 uL) was carefully injected into the sheath of LLCN and the muscles around "Tianquan" (PC 2) and bis Benzimide (bB, 5 uL) injected respectively into and the LICN and the stellate ganglion. Results : Following electric stimulation of LICN, the firing rates of LLCN and myoelectrical activity of"Tianquan" (PC 2) increased pronouncedly in comparison with pre- stimulation (P < 0 – 01); while no significant changes of myoelectric signals of "Tianfu" (LU 3) were found. After cutting off the dorsal and ventral roots of C7 – T2 separately, the thresholds of the discharges of the LLCN and "Tianquan" (PC 2) area responding to stimulation of the LIEN increased gradually, being significant in TI, C8 and C7 in comparison with pre-section of nerves. After microinjection of PI and bB, light microscopic examination showed that the single and the double labeled neurons were mainly located in C7, C8 and T, dorsal root ganglions with C8 being the utmost. Conclusion : a) There exists a relatively specific connection between " Tianquan" (PC 2) and the heart; b) C7 – T, segments may be responsible for the connection between "Tianquan"